Pseudogene MAPK6P4-encoded functional peptide promotes glioblastoma vasculogenic mimicry development
Journal: Communications Biology (2023)
Institution: Department of Neurobiology, School of Life Sciences, China Medical University, Shenyang, PR China
Research Areas: Cancer research
Cell Lines: U251, U373, and HEK 293T cells (human GBM cell lines, human embryonic kidney)
Summary: The paper studied the role of a functional peptide encoded by the pseudogene MAPK6P4 in promoting vasculogenic mimicry (VM) in glioblastoma (GBM), a highly aggressive malignant brain tumor. The study showed pseudogene MAPK6P4 deficiency represses VEGFR2 and VE-cadherin protein expression levels, as well as inhibits the proliferation, migration, invasion, and VM development of GBM cells. They also found that the MAPK6P4-encoded peptide, PEP, is expressed in GBM cells and promotes VM formation by activating the ERK1/2 signaling pathway, in addition, the PEP expression is associated with poor prognosis in GBM patients.The authors used HoloMonitor M4 to study the effects of PEP on GBM cell proliferation, migration, and in vitro VM formation ability.