Authors: Herman Huang et al.

Journal: Journal of Molecular and Cellular Cardiology (2024)

Research Areas: Cardiology

Cell Lines: primary rat cardiomyocytes, primary mouse cardiomyocytes (cardiomyocytes isolated from ventricle)

Summary: In this study, the authors use HoloMonitor M4 to evaluate the proliferative response of mononucleated and binucleated cardiomyocytes to a proliferative stimulus. By tracking long-term cultures at a single-cell level, they monitor cardiomyocyte dynamics in real-time. The findings demonstrate that CHIR99021 treatment promotes complete cell division in both mono- and binucleated cells, and reveal a link between cardiomyocyte size, nucleation, and cell cycle control. These insights give a better understanding of the cellular mechanisms underlying the loss of cardiac regenerative capacity.

Keywords: neonatal rat, heart, cardiomyocyte, cell division, nucleation, cell culture, digital holographic imaging