Customer Publication
Real-time morphometric analysis of targeted therapy for neuroblastoma cells in monolayer and 3D hydrogels using digital holographic microscopy
Journal: iScience (2024)
Research Areas: Cancer Research
Cell Lines: SK-N-BE (2), SH-SY5Y (Human neuroblastoma cell lines)
Summary: In this study, a gelatin- and silk fibroin-based hydrogel system with cross-linked vitronectin (VN) was used as an artificial biomimetic three-dimensional (3D) environment to mirror aggressive neuroblastoma (NB) tumors and tested for long-term cell response to Cilengitide (CLG). Using HoloMonitor M4, the authors showed that CLG caused cell detachment in monolayer cultures of MYCN-amplified SK-N-BE (2) and ALK-mutated SH-SY5Y human neuroblastoma cell lines. Cell detachment and aggregation were maintained in hydrogel-free monolayer cells whereas cells embedded in hydrogels presented different responses to treatment, suggesting differential anoikis resistance between the two cell lines.