Journal: Archives of Clinical and Biomedical Research (2022)
Institution: Université de Lyon
Research Areas: Cell research
Cell Lines: A549 (Human lung carcinoma cells)
Summary: Reduced glutathione (GSH) is a major intracellular antioxidant, and It plays important roles in many cellular processes, including cell differentiation, proliferation, and apoptosis. Authors demonstrated that extracellular concentration of GSH markedly increases cell volume within few hours, in a dose-response manner. Pre-incubation of cells with BSO, the inhibitor of γ-glutamylcysteine synthetase, responsible for the first step of intracellular glutathione synthesis, did not change the effect of reduced glutathione on cell volume, suggesting a mechanism limited to the interaction of extracellular reduced glutathione on cell membrane. Similarly, inhibition of γ-glutamylcyclotransferase involved in intracellular glutamate production, essential for intracellular production of glutathione, had no effect on the action of reduced glutathione. Oxidized glutathione exerted no effect on cell volume. Results show that GSH decreases cell adhesion resulting in an increased cell volume. Since many cell types are able to export GSH, the present results suggest that this could be a fundamental self-regulation of cell volume, giving the cells a self-control on their adhesion proteins.