Journal: bioRxiv (2022)
Institution: Université de Lyon, UMR Ecologie Microbienne
Research Areas: Cell research
Cell Lines: A549 (Human lung carcinoma cells,)
Summary: Glutathione is the most abundant thiol in animal cells. Reduced glutathione (GSH) is a major intracellular antioxidant neutralizing free radicals and detoxifying electrophiles. It plays important role in many cellular processes, including cell differentiation, proliferation, and apoptosis. In the present study, we demonstrate that extracellular concentration of reduced glutathione markedly increases cell volume within a few hours, in a dose-response manner. Pre-incubation of cells with BSO, the inhibitor of γ-glutamylcysteine synthetase, responsible for the first step in intracellular glutathione synthesis did not change the effect of reduced glutathione on cell volume suggesting a mechanism limited to the interaction of extracellular reduced glutathione on the cell membrane. Similarly, inhibition of γ-glutamylcyclotransferase involved in intracellular glutamate production had no effect on the action of reduced glutathione. Oxidized glutathione exerted no effect on cell volume. Results show that reduced GSH decreases cell adhesion resulting in an increased cell volume. Since many cell types are able to export GSH, the present results suggest that this could be fundamental self-regulation of cell volume, giving the cells self-control over their adhesion proteins. HoloMonitor M4 is used for cell morphology (cell volume) changes.