Customer Publication

Refractometry of Microscopic Objects With Digital Holography

Authors: Mats Gustafsson, Mikael Sebesta

Journal: Applied Optics (2004)

Institution: Phase Holographic Imaging PHI AB, Lund

Research Areas: Method development

Summary: Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.

Keywords: digital holographic imaging, effective refractive index, light intensity, phase contrast, phase imaging, refractive index, digital holography technology

Read the article …