Salinomycin Treatment Specifically Inhibits Cell Proliferation of Cancer Stem Cells Revealed by Longitudinal Single Cell Tracking in Combination with Fluorescence Microscopy
Applied Sciences (2020)
Institution: Lund University, Sweden
Cell Line: JIMT-1 (Human breast cancer cells)
Research Area: Cancer research
Conclusions: Cell lines derived from tumors are comprised of a mixture of cells, which have different phenotypes. Hence, in cell population-based assays, small sub-population impact or behavior against treatment can be undetected. In this study, S. Kamlund with colleagues has used HoloMonitor M4 in combination with fluorescence microscopy to study JIMT-1 breast cancer cells for an extended time and study Salinomycin effects on individual cells via longitudinal single-cell tracking. Authors have shown that Salinomycin specifically targets a CD24- cell subpopulation by inhibiting its proliferation and the surviving CD24+ cell subpopulation was more epithelial-like.