Authors: Róbert Alföldi, József Á. Balog, Nóra Faragó, Miklós Halmai, Edit Kotogány, Patrícia Neuperger, Lajos I. Nagy, Liliána Z. Fehér, Gábor J. Szebeni, László G. Puskás

Journal: Cells (2019)

Institution: University of Szeged

Research Areas: Cancer research

Cell Lines: A549 (Human lung adenocarcimona cell line)

Summary: In this study authors have characterized lung cancer markers from cells cultured in two-dimensional (2D) and three-dimensional (3D) fashion. They have concluded that 3D cell cultures should be used for drug screening as these structures are more similar to the in vivo tumor conditions. R. Alfödi et al have used phase contrast functionality in HoloMonitor M3 to evaluate different culture conditions on A549 cells after 4 and 9 days post seeding.

Keywords: HoloMonitor M3, phase contrast, single cell mass cytometry, single cell proteomics, non-small cell lung cancer, three-dimensional tissue culture