Subpopulations of Organoid-Forming Cells Have Different Motility

Authors: D. Jimenez et al.

Journal: Applied Sciences (2020)

Institution: Lund University

Research Areas: Organoid formation

Cell Lines: HN26 (p16+ human papillomavirus (HPV16+) cell line)

Summary: Cancer stem cells from oropharyngeal squamous cell carcinoma can self-renew and differentiate into heterogeneous 3D structures, i.e. organoids, which have tumor cell features. However, there is a need for easy-to-use methods to understand organoid formation. In this study, D.G. Jimenez with colleagues have used HoloMonitor M4 and digital holographic microscopy to study tumor organoid development from single cells. Authors have revealed organoid formation kinetics by studying cell aggregate morphology (area, volume, thickness) changes and single cell motility.

Keywords: HoloMonitor M4, cell morphology and cell movements, organoid formation; digital holography; cancer stem cells; oropharyngeal squamous-cell carcinoma

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