Authors: Paulina Czechowicz et al.

Journal: Cell Communication and Signaling (2024)

Institution: Department of Biophysics, Faculty of Biotechnology, University of Wroclaw, Wroclaw, Poland

Research Areas: Apoptosis Regulation

Cell Lines: HeLa S3, Calu3, 16HBE14o- (cancer and noncancer human airway epithelial cells (Calu 3 and 16HBE14o-, respectively))

Summary: Regulation of endoplasmic reticulum (ER) homeostasis plays a critical role in maintaining cell survival. When ER stress occurs, a network of three pathways called the unfolded protein response (UPR) is activated to reestablish homeostasis. miR-1244 serves as a regulatory check point for all 3 pathways and down regulation of miR-1244 contributes to the pro-survival arm of UPR. Based on cell morphology measurements, data from HoloMonitor M4 was used to determine cell viability after treatment with ER stress inducing substances and inhibitors affecting miR-1244.

Keywords: cell biology, protein-ligand interactions, receptors, cytokines and growth factors