TRPM2 Modulates Neutrophil Attraction to Murine Tumor Cells by Regulating CXCL2 Expression
Journal: Cancer Immunology, Immunotherapy (2018)
Institution: Hebrew University
Research Areas: Immunoncology
Cell Lines: 4Ti, AT3, TRPM2, LLC, human neutrophils (4T1 and AT3 mouse breast tumor cells, LLC=Lewis Lung Carcinova)
Summary: The tumor microenvironment in vivo is not limited to the tumor cells themselves but also includes many kinds of immune cells. In this study the researchers explored cytotoxic effects of neutrophils in a co-culture with murine tumor cells. To exert their cell killing effect the neutrophils must reach the target cells, i.e. tumor cells. Using the HoloMonitor cell motility and migration assays the scientists could explore how the ability of the neutrophils to approach the cancer cells was affected during various conditions. Their findings are also supported and visualized in an associated time-lapse movie.