Cell Morphology Assay

High-content.

Numerous results from one sample on single-cell level

Usually, we think of cell morphology as cell area, cell volume, and maybe cell shape. However, using HoloMonitor, you can analyze more than 30 different cell morphology features over time. Above all, many of those parameters are unique for our non-invasive digital holography technology. Now, this provides you with in-depth data on every single cell in your population. Furthermore, you can gain further results from the same sample by combining this assay with other HoloMonitor live cell assays or the add-on fluorescence unit. For example, you can extract cell proliferation and cell motility data for a more detailed picture.

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Accurate.

Exact cell segmentation makes live cell identification simple

Firstly, individual living cells are much more easily singled out from the background with quantitative phase imaging in comparison to traditional methods, such as phase contrast. As a result, robust computer algorithms can automatically and precisely quantify cell morphology changes down to every single cell in your population. This way, you receive exact cell morphology assay results. And measure what you actually want to study.

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Versatile.

Kinetic cell morphology is in the focus of many applications

Many naturally occurring cell processes, such as cell division, cell density, and cell movement, affect cell morphology. Also, combining cell morphology assay results with cell proliferation and cell motility is a powerful tool. Not only, for example, for cytotoxicity studies or in vitro drug development.

Not only can you detect early drug responses from cell morphology changes. But also study the cell cycle, analyze cell death kinetics, or classify subpopulations.

Individual cell morphology insights are useful for:

• For evaluation of cell culture health and quality
• As early and sensitive markers to detect drug effects
• To provide information on drug mode of action
• For identification of dying cells

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Continuous.

Never miss an important cellular event

HoloMonitor is designed to operate 24/7 directly inside your standard incubator. This makes it possible for you to continuously monitor and quantify your cell cultures in their undisturbed environment. The high temporal resolution of the system allows you to study in-depth cellular processes, cell behavior, and changing cell morphology characteristics in real-time without missing any important cellular events in your culture.

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