Advancing Cancer Research with Quantitative Live Cell Imaging
Cancer is a leading human disease with persistently high mortality rates. Thus, understanding the how, where, why and with whom metastatic cancer cells disseminate and proliferate is one of the highest importance in cancer research. The HoloMonitor live cell imaging system advances your cancer research with a non-invasive way to measure and quantify cell activity in real-time without compromising the integrity of your precious cells. Therefore, you can get more accurate and native results from your experiments.
A live cell analysis tool with real-time data
Find out more about how HoloMonitor live cell imaging can accelerate your cancer research!
Featured publications within cancer research
Compare directional cell movement in medulloblastoma cancer cells
The expression of long non-coding RNAs (lncRNAs) influences diverse cellular activities and is often cancer-specific. Thus lncRNAs are a useful diagnostic and therapeutic target for cancer. By altering the expression of lncRNA levels, it is possible to affect the cell movement in medulloblastoma cancer cells.
In this publication, the authors evaluated the effect of lncRNA on cell motility and showed that it promotes the migration of tumor cells using HoloMonitor wound healing assay and single cell tracking assay.
Measure mitotic activity in melanocytes
Cutaneous melanoma is deadly skin cancer. The activating mutation BARFV600E mutation exists both in moles and over half cutaneous melanomas.
In this study, in order to investigate why the same mutation has such different consequences in moles and melanomas, the authors using HoloMonitor studied cell proliferation, cell dry mass, cell death and cell growth rate. The experiments showed by increasing the levels of two microRNAs in melanocytes induces mitotic failure, genome duplication, and proliferation arrest. BRAFV600E induces a similar proliferation arrest in primary human melanocytes that is both reversible and conditional depending on the differentiation state of the melanocyte.
Quantify cell motility under hypoxia stress in breast cancer
Hypoxia is an important factor known to initiate the metastatic cascade in cancer, activating cell motility and invasion by silencing cell adhesion genes.
In the study, the authors using cell motility assay demonstrated that epigenetic enzyme (G9a) accumulation in hypoxia leads to CDH10 protein transcription repression. This enhances cancer cell motility and is an integral part of hypoxia-mediated epithelial-mesenchymal transition (EMT) in breast cancer.
Study cell movements in 3D culture for cancer biomechanics
Studying the tumor microenvironment is one of the highest importance in cancer research as most tumors depend on a supporting cast of non-cancerous cells for their nourishment, growth and survival. 3D cell culturing mimics the actual physiological conditions in vivo than the traditional 2D cell cultures. This is particularly important when studying cell movements as the higher complexity of the microenvironment affect the biomechanics.
The secrets to truly controlled cell experiments for cancer research
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Answer cancer research questions with quantitative live cell imaging
Browsing publications in cancer research
Get inspired by other research fellows, and learn how HoloMonitor quantitative live cell imaging can benefit your cancer research:
Simple and automatic monitoring of cancer cell invasion into an epithelial monolayer using label-free holographic microscopy
Journal: Scientific Reports (2022)
Research Areas: Cancer Research
Cell Lines: Vero, HeLa
Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response
Journal: Acta Biomaterialia (2022)
Research Areas: cancer research
Cell Lines: HepG2
Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination
Journal: International Journal of Biological Sciences (2022)
Research Areas: Cancer research
Cell Lines: SUM159PT, SUM149PT, MDAMB231, MCF7, BT474,, SKBR3
Journal: Haematologica (2022)
Research Areas: Leukemia
Cell Lines: MLL–AF9
Journal: Frontiers in Cell and Developmental Biology (2022)
Research Areas: Cancer research
Cell Lines: LNCaP C4-2, DU145
A New Class of PSMA-617-Based Hybrid Molecules for Preoperative Imaging and Intraoperative Fluorescence Navigation of Prostate Cancer
Journal: Pharmaceuticals (2022)
Research Areas: Oncology
Cell Lines: LNCaP, PC-3
Discover our partners in cancer research
Gladstone Institutes, UCSF and PHI jointly operate San Francisco’s Holographic Imaging Cytometry Center of Excellence.
Supported by PHI, Dr. Robert Judson-Torres and his team at Huntsman Cancer Institute aim to develop methods to quantify the phenomena known as epithelial-mesenchymal transition (EMT).
PHI and Malmö University have a long-standing collaboration, dating back to 2008. The collaboration has resulted in several peer reviewed publications and a doctoral thesis.
The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis.