Cell Counter

Your automatic cell counter

HoloMonitor cell count assay icon

The HoloMonitor® cell counter is designed for automated cell counting of suspension cells. It offers a no-hassle, high-precision automatic cell counter with results presented within a minute. Count live and/or dead cells in your cell culture and can be used not only for seeding but also for end-point cell counting of suspension cells.


Why choose the HoloMonitor Cell Counter?


Accurate cell count number for accurate and repetitive cell-based assay.

Learn more…


Automatic cell counting to speed up your experiment but maintain accuracy.

Learn more…


Multiple results from one sample with this 8-in-1 live cell analysis tool.

Learn more…

Assay Output

What results do you get with this cell counting system?

The HoloMonitor Cell Counter is designed for automated cell counting of suspension cells. It can be used in two ways, either to count cells as a preparation for seeding, or for endpoint cell counting of suspension cells where data of cell volume and area is acquired.

When used for seeding, a dilution calculator can provide the volume needed for sampling. When used as endpoint cell counter, the created distribution graphs based on cell volume and area are provided. Certainly, our App Suite software provides the opportunity to export all data as Excel files (.xlsx) for further analysis.

Endpoint data are given per well:

  • Cell count (cells/ml)
  • Mean cell volume (µm3)
  • Mean cell area (µm2)
  • Distribution graphs on cell area and volume
HoloMonitor App Suite cell counter assay result screen
HoloMonitor App Suite cell counter assay dilution calculator
With our cell counter, you also automatically receive cell a dilution calculator.

Do you want to count adherent cells as well?


Quick and accurate cell count in one minute

Cell counting is a routine task for any researcher working with cells, but counting cells manually with conventional hemocytometers is tedious. It is not only time-consuming but also requires scrutiny. However, obtaining accurate cell counts is important for both cell expansion and for accurate and repetitive results for cell-based assays. Besides, HoloMonitor auto-focus leads to highly reproducible cell counts with reduced user-to-user variability compared to a hemocytometer and cell counters with manual focus. 

To the top

Key Applications

What can you study with this automatic cell counter?

Cell QC

Suspension cell morphology

Cell expansion

Cell seeding


Minimized the workload for you

Undoubtedly, automated cell counting can certainly speed up your workflow and eliminate user-to-user differences. The HoloMonitor Cell Counter is designed to count suspension cells using a non-grid counting chamber automatically. Cell count is provided in a number of cells per volume unit, i.e., the same principle as with Bürker chamber cell counting. In addition, a dilution calculator tool will automatically provide the stock suspension volume needed for seeding the cells in the upcoming experiment or next passage.

To the top

Cell Counter Protocol

How do you set up this cell counter?

Cell counting slides mounted on the HoloMonitor slide holder

1. Sample preparation

Simply add cell suspension to the counting chamber. Then, slide the counting chamber into the vessel holder onto the HoloMonitor stage.

The HoloMonitor M4 cell culture microscope on a lab bench next to cell culture accessories

2. Assay setup

Select Cell Counter and follow the guided setup. Next, select your imaging positions. Start imaging and walk away!

3. Explore results

Review results determined automatically by the software, and re-analyze the data if you want.


Much more than just a cell counting system

Many experiments today require not only cell count numbers but also several key cell features. HoloMonitor Cell Counter, on the other hand, provides much more than just cell count. We also provide information and graphs showing basic cell morphology are part of the automatic result presentation in the cell counter assay such as cell diameter and volume and provide distribution graphs based on these parameters.

To the top

The live cell imaging method not only saves time and labor, but also provides more accurate and detailed information.

Huang J, GUO P & MOSES M
Boston children’s hospital

Browse our publication library for more references and see how our worldwide HoloMonitor customers use this cell counting system.

Propofol prevents oxidative stress and apoptosis by regulating iron homeostasis and targeting JAK/STAT3 signaling in SH-SY5Y cells

Authors: Ying Zhang et al.

Journal: Brain Research Bulletin (2019)

Research Areas: Neuroscience

Cell Lines: SH-SY5Y

Keywords: HoloMonitor M4, Cell count, cell morphology, Propofol, Oxidative stressIron metabolism, Apoptosis, Inflammation, JAK/STAT3 signaling, Neurodegenerative disorders

Learn more …

Lipopolysaccharide promoted proliferation and adipogenesis of preadipocytes through JAK/STAT and AMPK-regulated cPLA2 expression

Authors: Chao-Chien Chang et al.

Journal: International Journal of Medical Sciences (2019)

Research Areas: Cell research, Inflammation

Cell Lines: 3T3-L1

Keywords: HoloMonitor M4, Cell count, Proliferation, PLA2, Lipopolysaccharide, Adipocyte, Adipogenesis, Obesity

Learn more …

Translational medicine in pulmonary-tenal crosstalk: Therapeutic targeting of p-cresyl sulfate triggered nonspecific ROS and chemoattractants in dyspneic patients with uremic lung injury

Authors: Jia-Feng Chang et al.

Journal: J. Clin. Med. (2018)

Research Areas: Cancer research

Cell Lines: A549

Keywords: HoloMonitor M4, Cell counting, P-Cresyl sulfate, Uremic lung Injury

Learn more …

CAT3, a prodrug of 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro[9,10-b]-indolizidine, circumvents temozolomide-resistant glioblastoma via the Hedgehog signaling pathway, independently of O6-methylguanine DNA methyltransferase expression

Authors: Ji M et al.

Journal: OncoTargets and Therapy (2018)

Research Areas: Cancer research

Cell Lines: U251/TMZ and T98G

Keywords: HoloMonitor M4, Cell count, Gli inhibitor, Chemotherapy, Lomeguatrib, Xenograft tumor model

Learn more …