Advancing Drug Discovery & Development with Quantitative Live Cell Imaging
Drug discovery and development is a lengthy and expensive process, and cell-based drug screenings play an important role in basic research. HoloMonitor is designed for automated and detailed analysis of drug-dose responses in adherent cells. It uses non-invasive quantitative phase imaging (QPI) to identify cells which enables direct measurement of drug dose-response curves over time. In this way, you get accurate and reliable data without disturbing your cells.
A label-free live cell analysis tool
Find out more how the HoloMonitor live cell imaging system is used in in vitro drug discovery & development!
Identify off-target effects of metformin
In recent years, identifying the different targets for already licensed drugs has gained importance. Metformin, an oral antidiabetic drug, might have other clinical use in various diseases.
Thus, the off-target effects of metformin were identified in this study. The authors used HoloMonitor cell morphology assay to measure cell area, cell volume, average thickness, and irregular parameters for cells treated with metformin.
Quantify cytotoxicity of the combination nanoparticles for drug treatment
In this publication, the authors showed that the codelivery of C6 ceramide and dox using a liposomal platform significantly correlates with an antiproliferative effect, which is due to the cell cycle regulation, and subsequent induction of apoptosis. The apoptosis event was quantified by measuring the decrease in the cell-occupied area using HoloMonitor cell morphology assay.
Visualize the cell change in targeted drug delivery
In order to simultaneously target bulk cancer cells to prevent cancer growth and metastases, researchers are developing different target drug delivery systems to improve the biodistribution and solubility properties of nano-carries in drug deliveries.
In this study, the authors have used monoclonal antibody-modified immunoliposomes for the targeted delivery of paclitaxel and salinomycin for cancer therapy. By using HoloMonitor, the authors measured the cell morphology, proliferation and cell division changes between treatments. The results confirmed the efficiency of the antibody-targeted liposomal preparation in the therapy of cancer by giving significantly better cellular division arrest profiles and cell death.
On-demand webinar
The secrets to truly controlled cell experiments for in vitro drug development
Discover HoloMonitor Live Cell Assays
Learn more about what you can do with HoloMonitor
Kinetic motility assay
Single cell tracking 
Cell morphology assay
Wound healing assay
Dose response assay Realiable drug-response results with quantitative live cell imaging
The time-lapse videos show L929 cells untreated and drug-treated with 10 µM etoposide.
The time-lapse video shows the Cama-1 breast cancer cell line, which was treated with a cytotoxic compound.
Browsing publications in drug discovery
Get inspired by other research fellows, and learn how HoloMonitor quantitative live cell imaging can benefit your drug discovery and development:
Enhanced Cytotoxicity of Folic Acid-targeted Liposomes Co-loaded With C6 Ceramide and Doxorubicin: in Vitro Evaluation on HeLa, A2780-ADR and H69-AR Cells
Journal: Molecular pharmaceutics (2015)
Research Areas: Cancer research, Drug research
Cell Lines: HeLa
Targeted Tumor Therapy by Rubia Tinctorum L.: Analytical Characterization of Hydroxyanthraquinones and Investigation of Their Selective Cytotoxic, Adhesion and Migration Modulator Effects on Melanoma Cell Lines
Journal: Cancer cell international (2015)
Research Areas: Cancer research, Drug research
Cell Lines: A2058, HT168-M1, MRC-5
Poloxamer Bioadhesive Hydrogel for Buccal Drug Delivery: Cytotoxicity and Trans-epithelial Permeability Evaluations Using Tr146 Human Buccal Epithelial Cell Line
Journal: International journal of pharmaceutics (2015)
Research Areas: Drug research
Cell Lines: Tr146
Interfacing Antibody-based Microarrays and Digital Holography Enables Label-free Detection for Loss of Cell Volume
Journal: Future science oa (2015)
Research Areas: Cancer research, Drug research
Cell Lines: Jurkat, U2932
Environmental and Toxicological Impacts of Glyphosate With Its Formulating Adjuvant
Journal: International Journal of Agricultural, Biosystems Science and Engineering (2014)
Research Areas: Drug research
Cell Lines: NE-4C
Edaravone Protects Against Methylglyoxal-induced Barrier Damage in Human Brain Endothelial Cells
Journal: PLOS ONE (2014)
Research Areas: Drug research
Cell Lines: hCMEC/D3
With the HoloMonitor M4 we are able to easily distinguish different cell types by their unique size, shape, and movement patterns. We can’t wait to see what new biology we will discover with this innovative approach!
Alexander Payumo
San Jose State University