HoloMonitor publication highlights 2024

The paper describes the effects of Spinosyn A, a natural insecticide, on breast cancer cells. The authors used HoloMonitor M4 to monitor the effects of Spinosyn A (SPA) on breast cancer cells with cell morphology, proliferation and motility.

The results revealed that the cells are increasing in volume in a concentration-dependent manner when treated with SPA. Single-cell tracking of cell families showed that the cells exhibited longer division times, and their motility was reduced when exposed to SPA. Further morphological analysis reveals that SPA treatment increased shape roughness due to SPA exposure, which is associated with increased apoptosis. On the other hand, irregularity and eccentricity are both decreasing in a concentration-dependent manner. Since cancer cells are highly irregular, a decrease in irregularity suggests that T47-D cells become less cancerous when treated with SPA for 24 hours.

The article investigates how increased cell size and nucleation influence the outcomes of cardiomyocyte cell division in vitro.

In this study, the authors use HoloMonitor M4 to evaluate the proliferative response of mononucleated and binucleated cardiomyocytes to a proliferative stimulus. By tracking long-term cultures at a single-cell level, they monitor cardiomyocyte dynamics in real-time. The findings demonstrate that proliferative stimulus promotes complete cell division in both mono- and binucleated cells, and reveal a link between cardiomyocyte size, nucleation, and cell cycle control. These insights give a better understanding of the cellular mechanisms underlying the loss of cardiac regenerative capacity.

In this study, the researchers utilize digital holographic microscopy and quantitative phase imaging to uncover morphological, optical, and behavioral distinctions between melanocytes and SK-MEL-28 melanoma cells, without the need for labels or any other damaging interventions.

Advanced techniques, including PCA and t-SNE, reveal significant differences in size, shape, optical properties, and migration patterns. These insights support the identification of melanoma biomarkers, enhancing diagnostic precision and therapeutic strategies in melanoma research. HoloMonitor M4 was used to study the morphology and movement between melanocytes and SK-MEL-28 melanoma cells.

We at PHI are especially thrilled that this publication was featured on the cover of Advanced Biology, showcasing images of melanocytes and melanoma cells captured with the HoloMonitor M4. Well done, Farhat and colleagues!