HoloMonitor publication highlights 2023

In 2023, cell researchers all over Europe, the USA, and Asia continued to show the versatility of HoloMonitor in their innovative research. We’re nearing 300 peer-reviewed publications featuring HoloMonitor data — a proud milestone for us at PHI.

This year, HoloMonitor data contributed to diverse fields, from cancer research to immunology. As a helpful tool for studying cell behavior, its label-free Cell Morphology Assay remains a favorite among our users. At the same time, applications like Single-cell tracking and Kinetic cell motility have further gained popularity.

In this blog, we present the researchers behind these studies and delve into the HoloMonitor publication highlights 2023 that unlock our cells’ secrets…

Keep on reading!

Below, we highlight a few notable articles from different research fields
that feature HoloMonitor and its range of software assays.

Cell Communication and Signaling (2023)

IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A

Gebert, Magdalena et al., Medical University of Gdansk, Gdansk, Poland

The article investigates the role of IRE1, a key sensor of the unfolded protein response (UPR), in regulating microRNA (miRNA) expression during endoplasmic reticulum (ER) stress.

The authors use next-generation sequencing (NGS) and nuclear-cytosolic fractionation to show that IRE1 degrades two specific pre-miRNAs, pre-miR-301a and pre-miR-106b, in the nucleus during ER stress. Also, they identify a novel target of miR-301a-3p, a pro-apoptotic UPR factor called GADD45A. They demonstrate that blocking miR-301a-3p binding to GADD45A mRNA increases GADD45A expression and promotes cell death. They conclude that IRE1 has a dual role in the UPR, as it can splice XBP1 mRNA for survival or degrade pre-miR-301a to induce apoptosis.

The authors use HoloMonitor to measure the cell viability and cell morphology of human bronchial epithelial cells under different conditions of ER stress and miRNA modulation. They show that HoloMonitor can detect changes in cell number, cell area, cell volume, and optical cell thickness in response to ER stress and miRNA manipulation (see Cell Morphology Assay). They also use HoloMonitor to monitor the dynamics of cell death over time and to quantify the percentage of apoptotic cells.

Keywords: cell morphology, microRNA, cell viability, cell biology, protein-ligand interactions, receptors, cytokines, growth factors, Cell Lines: 16HBE14o (human bronchial epithelial)

Have you followed our #mondayreads on LinkedIn?

Our scientist team invites you to follow along with our publications highlights on LinkedIn on Mondays. There, we present the latest peer-reviewed publications featuring HoloMonitor data. See you online!

Communications Biology (2023)

Pseudogene MAPK6P4-encoded functional peptide promotes glioblastoma vasculogenic mimicry development

Zhang, Mengyang et al., China Medical University, Shenyang, China

The paper studied the role of a functional peptide encoded by the pseudogene MAPK6P4 in promoting vasculogenic mimicry (VM) in glioblastoma (GBM), a highly aggressive malignant brain tumor.

The study showed pseudogene MAPK6P4 deficiency represses VEGFR2 and VE-cadherin protein expression levels. In addition, it inhibits the proliferation, migration, invasion, and VM development of GBM cells. Furthermore, they found that the MAPK6P4-encoded peptide, PEP, is expressed in GBM cells and promotes VM formation by activating the ERK1/2 signaling pathway. In addition, the PEP expression is associated with poor prognosis in GBM patients.

The authors use HoloMonitor (see Single Cell Tracking Assay) to study the effects of PEP on glioblastoma cell proliferation, cell migration, and in vitro vasculogenic mimicry formation ability.

Keywords: glioblastoma, single cell tracking, CNS cancer, phosphorylation, cell migration, vasculogenic mimicry, Cell Lines: U251, U373, HEK 293T cells (human glioblastoma cell lines, human embryonic kidney)

Scatter plot of L929 etoposid treated cells

HoloMonitor Cell Morphology

We might think of cell morphology as cell area, volume, and maybe shape. However, using HoloMonitor, you can analyze more than 30 different cell morphology features over time. Above all, many of those parameters are unique for label-free QPI imaging. You gain detailed data on every single cell in the population. Furthermore, you can create additional results from the same sample using the other HoloMonitor live cell assays or the add-on fluorescence unit. Learn more in our free cell webinar!

Cells (2023)

Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells

Berlin, Frida et al., Lund University, Lund, Sweden

Learn more about Frida’s research in our HoloMonitor user webinar series!

The article investigates the role of mast cell tryptase in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation.

The study found that mast cell tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell division intervals. The elevated cell growth induced by tryptase remained in a pro-inflammatory state. Tryptase also increased the expression of the anti-apoptotic protein BIRC3, as well as growth factor release in epithelial cells.

The authors use HoloMonitor (see Cell Proliferation Assay) to investigate the cell growth and cell division effects of mast cell tryptase on bronchial and alveolar epithelial cells.
The authors performed a thorough and detailed mast cell growth analysis, comparing the cell growth of untreated and tryptase-treated cells. Images were captured every 15 minutes over 72 hours, generating 288 images per focus position and over 2000 images per well and per repeat.

Keywords: cell proliferation, mast cell, proteases, tryptase, bronchial epithelium, alveolar epithelium, growth factors, Cell Lines: BECs, BEAS-2B, AECs, A549 (Human bronchial epithelial cells, human alveolar epithelial cells)

Enriching Publications with Live Cell Videos

HoloMonitor offers high-quality images and videos. Coupled with detailed quantitative data down to a single-cell level, this provides a comprehensive view of cell behavior. This multifaceted data presentation not only enhances the understanding but also strengthens the scientific findings from various perspectives. And it’s beautiful, isn’t it?

There are many amazing HoloMonitor videos featured in this Toxicology Reports publication titled “A New Animal Product Free Defined Medium for 2D and 3D Culturing of Normal and Cancer Cells to Study Cell Proliferation and Migration as Well as Dose Response to Chemical Treatment.”

JIMT-1 cells imaged every 5 minutes for 72 hours

JIMT-1 spheroid cell migration imaged for 72 hours

IK Channel-Independent Effects of Clotrimazole and Senicapoc on Cancer Cells Viability and Migration

Authors: Paolo Zuccolini et al.

Journal: International Journal of Molecular Sciences (2023)

Research Areas: Cancer Research

Cell Lines: WM266-4, panc-1

Keywords: HoloMonitor M4, wound healing assay, ik, kca3, 1, kcnn4, cancer, melanoma, pancreatic duct adenocarcinoma (pdac), blockers, clotrimazole, senicapoc

IRE1-mediated degradation of pre-miR-301a promotes apoptosis through upregulation of GADD45A

Authors: Gebert et al.

Journal: Cell Communication and Signaling (2023)

Cell Lines: 16HBE14o

Keywords: HoloMonitor M4, cell morphology assay, cell viability, cell biology, protein-ligand interactions, receptors, cytokines and growth factors

Expression profiles and function prediction of tRNA-derived fragments in glioma

Authors: Wei et al.

Journal: BMC Cancer (2023)

Research Areas: Cancer research

Cell Lines: U87MG, U373MG, U251MG, SVG p12

Keywords: HoloMonitor M4, glioblastoma, single cell analysis, Glioma, tRNA-derived fragment, tRF, Non-coding RNA, S100A11

Pseudogene MAPK6P4-encoded functional peptide promotes glioblastoma vasculogenic mimicry development

Authors: Zhang et al.

Journal: Communications Biology (2023)

Research Areas: Cancer research

Cell Lines: U251, U373, and HEK 293T cells

Keywords: HoloMonitor M4, single cell tracking, cns cancer, phosphorylation, glioblastoma, vasculogenic mimicry

Mast Cell-Mediated Orchestration of Airway Epithelial Responses in Chronic Respiratory Diseases

Authors: Frida Berlin

Journal: Doctoral thesis (2023)

Research Areas: Cell biology

Keywords: HoloMonitor M4, Mast cells, proteases, tryptase, chymase, chronic respiratory diseases, asthma

A novel peptide P1-121aa encoded by STK24P1 regulates vasculogenic mimicry via ELF2 phosphorylation in glioblastoma

Authors: Li et al.

Journal: Experimental Neurology (2023)

Research Areas: Cancer research

Cell Lines: U251, U373, HEK293T, HA

Keywords: HoloMonitor M4, Single cell tracking, glioblastoma STK24P1, P1-121aa, ELF2 Phosphorylation

Polysaccharide-based nano-engineered multilayers for controlled cellular adhesion in label-free biosensors

Authors: Wasilewska et al.

Journal: International Journal of Biological Macromolecules (2023)

Research Areas: Materials Science

Cell Lines: MC3T3-E1

Keywords: HoloMonitor M4, Cell proliferation, Cell morphology, Macroion multilayers, Polysaccharide layers, Label-free biosensors, Streaming potential, OWLS, Resonant waveguide grating, Cell adhesion, Antimicrobial coatings, QCM

Induction of Fungal Secondary Metabolites by Co-Culture with Actinomycete Producing HDAC Inhibitor Trichostatins

Authors: Gwi Ja Hwang et al.

Journal: Journal of microbiology and biotechnology (2023)

Research Areas: Microbiology

Cell Lines: MDA-MB-231 cells

Keywords: HoloMonitor M4, Single cell tracking, co-culture system, fungal cryptic secondary metabolites, structure determination, physical interaction

HoloMonitor - A cell culture microscope directly inside your incubator

A live cell analysis system inside your incubator

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