Accelerate your research with
Quantitative Live Cell Imagin
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The HoloMonitor live cell imager viewed from the front

Meet HoloMonitor®

A Non-Invasive Incubator Microscope

  • Measure at once 30+ different cellular features from cell population down to single-cell level
  • Non-invasive imaging, directly in your incubator using standard cell culture vessels and formats
  • Easy and comprehensive presentation of real-time data and high-quality time-lapse videos
  • Easy-to-use, guided software that lets you reanalyse your experiments for more results

A Proven Technology

Holographic microscopy principle

QPI imaging with digital holography

The label-free HoloMonitor live cell imaging system is based on the principle of quantitative phase imaging (QPI). Right inside your standard incubator, HoloMonitor enables intuitively non-invasive, real-time visualization and precise quanti­fication of living cells without compro­mising cell integrity.

Segmentation of cells in a quantitative phase image

Simpler Live Cell Identification

Your imaged cells appear as well-separated peaks from the background in a quanti­tative phase image. This way, HoloMonitor’s sophisticated software can automatically and robustly segment individual cells — of various confluency, shapes and sizes.

The Applications

Kinetic Cell Morphology
(with 30+ parameters)

In-depth Single Cell
Tracking & Analysis

Kinetic Cell Motility &
Migration

Wound Healing
(Scratch assay)

Chemotaxis

Kinetic Cell Proliferation

Cell Growth | Cell Division
Cell Cycle

Cellular Differentiation

Kinetic
Drug Dose-Response

Cytotoxicity & Cell Death

Cell Counter

Cell Quality Control (QC)

Which application fits you most?
Find out how HoloMonitor can accelerate your research!

Multiple results from one experiment

Reanalyse your data with different HoloMonitor assays

Monica Hellesvik, University of Bergen

The labs were still closed, and no additional experiments could be performed. Thus, the solutions had to come from our respective home offices. And here, the HoloMonitor’s built-in software solution showed its strength with a variety of applications and gave me the opportunity to re-analyze previous image material from home. New discoveries were made that strengthened our previous results.

Monica Hellesvik, Arnesen Lab, University of Bergen
Research from home blog post

New Application Notes

Publications

KLF2+ stemness maintains human mesenchymal stem cells in bone regeneration

Authors: Y. Zhou et al.
Journal: Stem Cells (2019)
Research Areas: Stem cell therapy
Using HoloMonitor M4, Y. Zhou with colleagues, in real-time, have shown that the absence of zinc finger proteins influences mesenchymal stem cell motility speed and distance.

Read more …

Digital Holographic Imaging as a Method for Quantitative, Live Cell Imaging of Drug Response to Novel Targeted Cancer Therapies

Authors: L. Croft et al.
Journal: Theranostics: Methods and Protocols (2019)
Research Areas: Cancer research
Authors have investigated the use of HoloMontor M4 in cytotoxicity studies by evaluating drug-response effects on cell viability and proliferation, also employing single-cell tracking and population-wide analysis of multiple cell morphology parameters.

Read more …

Find out how other researchers use HoloMonitor:

Digital holographic microscopy: a noninvasive method to analyze the formation of spheroids

Authors: Zahra El-Schich
Journal: BioTechniques (2021)
Research Areas: Cancer research
In this study, Z. El-Schich describes a novel methodology utilizing digital holographic microscopy with HoloMonitor M4 for the analysis of spheroids.

Read more …

Label-Free Classification of Apoptosis, Ferroptosis and Necroptosis Using Digital Holographic Cytometry

Authors: K. Barker et al.
Journal: Applied Sciences (2020)
Research Areas: Basic Cell Research
In this publication, K.L. Barker with colleagues has aimed to develop a label-free, live-cell imaging-based assay for classifying forms of programmed cell death with single-cell resolution. By employing HoloMonitor M4 and studying cell morphology parameter changes, authors have developed a method, which with high accuracy could label-free classify cell death pathways in melanoma cells.

Read more …

Stanford University logo

The HoloMonitor M4 is a beneficial device for live-cell imaging, providing a lot of data. The good thing is that you can analyze your experiment using one software assay, and later, you can rerun the recorded data in another assay, which allows you to get the maximum from your experiment. I like it and would recommend it to all people doing a cytotoxicity assessment based on cell proliferation and optical thickness of the adherent unstained cells.

Sasa Vasilijic, PhD
Stanford University