Stem Cell Research

Advancing Stem Cell Research with Quantitative Live Cell Imaging

Stem cells are extremely sensitive and vulnerable to fluorescent labeling or strong laser power. The HoloMonitor live cell imaging system uses non-invasive digital holography to monitor stem cells without the need for any toxic labeling or staining. Therefore, you can image your precious stem cells for as long as you want in their native state.

HoloMonitor - A cell culture microscope directly inside your incubator

A label-free live cell analysis tool

Find out how HoloMonitor can accelerate your stem cell research!

Quantify the morphological and migratory characteristics in MSCs

HoloMonitor is a novel live cell analysis tool and provides accurate quantification of morphological and migratory characteristics including cell area, thickness, migration speed and distance.

In this study, the authors compared human periosteum and donor-matched iliac crest bone marrow multipotential stromal cell (BM MSC) using the HoloMonitor single cell tracking and kinetic morphology assay. Also, they use the data to define the development of novel bone graft substitutes to be repopulated by MSCs.

Real-time migration assay with HUVECs and KLF2 hMSCs

Study synergistic effects between KLF2+ hMSCs and HUVECs in bone regeneration

Mesenchymal stem cells (MSCs) are undifferentiated stem cells with the potential to differentiate into multiple lineages and are widely used as cell sources in bone tissue engineering. In addition, the co-culture of MSCs with endothelial cells (ECs) has attracted a great deal of interest in the development of strategies to achieve vascularized bone in recent years.

In this publication, the authors identified Krüppel-like Factor 2 (KLF2) as a marker of MSCs related to stemness. Moreover, the results showed that KLF2+ MSCs promoted angiogenesis of human umbilical vein ECs (HUVECs), influenced its migration speed via secretion of angiogenic factors and showed an osteogenic differentiation effect.

Study osteoporosis and adipogenesis

Understanding the processes of osteogenesis
and adipogenesis of the bone marrow is remarkably important in stem cell research.

In order to get a deeper insight into the delicate equilibrium between osteogenic and adipogenic differentiation and trans-differentiation at the population level, the authors use HoloMonitor to extract quantitative biological state indicators including the average thickness (T), and the roughness kurtosis (RK) to produce a scatter plot with full osteoblasts, full adipocytes and undifferentiated hBM-MSC. Then, this T-RK plot could be in principle generalized and exploited to evaluate at a glance the effect of specific treatments or culture conditions on hBM-MSC differentiation and osteo-adipo homeostasis.

On-demand webinar

The secrets to truly controlled cell experiments for stem cell research

Learn more about why non-invasive live cell imaging is perfect for today’s stem cell research.

Discover HoloMonitor Live Cell Assays

Learn more about what you can do with HoloMonitor

Enable stem cell research with quantitative live cell imaging

The 48-hour time-lapse shows differentiating human induced pluripotent stem (iPS) cells.

Time-lapse of stem cells grown on microgrid arrays from Micro surfaces.

Get inspired by other research fellows, and learn how HoloMonitor quantitative live cell imaging can benefit your stem cell research:

KLF2+ stemness maintains human mesenchymal stem cells in bone regeneration

Authors: Ying Zhou et al.

Journal: Stem Cells (2019)

Research Areas: Stem cell research

Cell Lines: hMSC, HUVEC

Keywords: HoloMonitor M4, cell movement and wound healing, angiogenesis, endothelial cell, KLF2, mesenchymal stem cell, osteogenesis, stemness, stem cell therapy

Learn more …

A Biophysical Approach to Quantify Skeletal Stem Cells Trans-differentiation as a Model for the Study of Osteoporosis

Authors: L. Petecchia et al.

Journal: Biophysical Chemistry (2017)

Research Areas: Stem cell research

Cell Lines: hBM-MSCs

Keywords: HoloMonitor M3, Quantitative phase imaging, Real time qPCR, Morphometry, Osteoblast, Adipocyte

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Interleukin-6 blockade attenuates lung cancer tissue construction integrated by cancer stem cells

Authors: Hiroyuki Ogawa et al.

Journal: Scientific Reports (2017)

Research Areas: Cancer research

Cell Lines: A549

Keywords: HoloMonitor M4, Cell motility, Cancer stem cell

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Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation

Authors: L. Petecchia et al.

Journal: Experimental Cell Research (2017)

Research Areas: Materials of Science

Cell Lines: hBM-MSC

Keywords: HoloMonitor M3, Cell morphology, Stem cell differentiation, Osteogenic markers, Biocompatibility, Biomaterial, Holographic microscopy, Implantation

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Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy

Authors: Tomoyuki Kawase et al.

Journal: International Journal of Radiation Biology (2016)

Research Areas: Stem cell research

Cell Lines: MSC-AT, MSC-BM

Keywords: Holomonitor M4, Cell morphology, Cell volume, Holographic microscopy, Mesenchymal stem cells, Periosteal cells, γ-ray irradiation, stem cell therapy

Learn more …

With the HoloMonitor M4 we are able to easily distinguish different cell types by their unique size, shape, and movement patterns. We can’t wait to see what new biology we will discover with this innovative approach! 

Alexander Payumo
San Jose State University