Advancing Stem Cell Research with Quantitative Live Cell Imaging
Stem cells are extremely sensitive and vulnerable to fluorescent labeling or strong laser power. The HoloMonitor live cell imaging system uses non-invasive digital holography to monitor stem cells without the need for any toxic labeling or staining. Therefore, you can image your precious stem cells for as long as you want in their native state.
A label-free live cell analysis tool
Find out how HoloMonitor can accelerate your stem cell research!
Quantify the morphological and migratory characteristics in MSCs
HoloMonitor is a novel live cell analysis tool and provides accurate quantification of morphological and migratory characteristics including cell area, thickness, migration speed and distance.
In this study, the authors compared human periosteum and donor-matched iliac crest bone marrow multipotential stromal cell (BM MSC) using the HoloMonitor single cell tracking and kinetic morphology assay. Also, they use the data to define the development of novel bone graft substitutes to be repopulated by MSCs.
Study synergistic effects between KLF2+ hMSCs and HUVECs in bone regeneration
Mesenchymal stem cells (MSCs) are undifferentiated stem cells with the potential to differentiate into multiple lineages and are widely used as cell sources in bone tissue engineering. In addition, the co-culture of MSCs with endothelial cells (ECs) has attracted a great deal of interest in the development of strategies to achieve vascularized bone in recent years.
In this publication, the authors identified Krüppel-like Factor 2 (KLF2) as a marker of MSCs related to stemness. Moreover, the results showed that KLF2+ MSCs promoted angiogenesis of human umbilical vein ECs (HUVECs), influenced its migration speed via secretion of angiogenic factors and showed an osteogenic differentiation effect.
Study osteoporosis and adipogenesis
Understanding the processes of osteogenesis
and adipogenesis of the bone marrow is remarkably important in stem cell research.
In order to get a deeper insight into the delicate equilibrium between osteogenic and adipogenic differentiation and trans-differentiation at the population level, the authors use HoloMonitor to extract quantitative biological state indicators including the average thickness (T), and the roughness kurtosis (RK) to produce a scatter plot with full osteoblasts, full adipocytes and undifferentiated hBM-MSC. Then, this T-RK plot could be in principle generalized and exploited to evaluate at a glance the effect of specific treatments or culture conditions on hBM-MSC differentiation and osteo-adipo homeostasis.
The secrets to truly controlled cell experiments for stem cell research
Discover HoloMonitor Live Cell Assays
Learn more about what you can do with HoloMonitor
Enable stem cell research with quantitative live cell imaging
Browsing publications in stem cell research
Get inspired by other research fellows, and learn how HoloMonitor quantitative live cell imaging can benefit your stem cell research:
A Biophysical Approach to Quantify Skeletal Stem Cells Trans-differentiation as a Model for the Study of Osteoporosis
Journal: Biophysical Chemistry (2017)
Research Areas: Stem cell research
Cell Lines: hBM-MSCs
Journal: Scientific Reports (2017)
Research Areas: Cancer research
Cell Lines: A549
Biophysical characterization of nanostructured TiO2 as a good substrate for hBM-MSC adhesion, growth and differentiation
Journal: Experimental Cell Research (2017)
Research Areas: Implantation
Cell Lines: hBM-MSC
Non-invasive, quantitative assessment of the morphology of γ-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy
Journal: International Journal of Radiation Biology (2016)
Research Areas: Stem cell therapy
Cell Lines: MSC-AT, MSC-BM