Study Cell Proliferation With
Quantitative Live Cell Imaging

cells quantitative cell imaging

Reuse cells

to save your time and money

multiple assay quantitative cell imaging

Multiple assays

from one single cell sample

Results quantitative cell imaging

Accurate results

with direct cell count

Why Quantitative Cell Imaging Over MTT Assay?

M4 sketch

Live Cell Imaging

  • Label-free
  • Direct cell count
  • No preparation needed
  • Real-time assay
  • Single-cell resolution
  • Quantitative

MTT assay

  • Cytotoxic
  • Indirect metabolism activity
  • Reagent preparation needed
  • Endpoint assay
  • Only population-level data
  • Qualitative
The HoloMonitor live cell imager viewed from the front

Meet HoloMonitor®

Our Live Cell Imaging Microscope

  • Label-free and high automation
  • More biological relevant and less labor-intensive
  • Real-time monitoring directly from the incubator
  • Robust system with standard cell culture vessels

Use your cells for more than proliferation

HoloMonitor’s cell-friendly nature makes it an ideal tool for viewing and analyzing live cell cultures. All HoloMonitor live cell assays are label-free, reducing the risk of unwanted toxicity and allowing cell samples to be reused. Hence, HoloMonitor saves your time and money. But foremost, save hard-to-get cells.

Stanford University logo

The HoloMonitor M4 is a beneficial device for live-cell imaging, providing a lot of data. The good thing is that you can analyze your experiment using one software assay, and later, you can rerun the recorded data in another assay, which allows you to get the maximum from your experiment. I like it and would recommend it to all people doing a cytotoxicity assessment based on cell proliferation and optical thickness of the adherent unstained cells.

Sasa Vasilijic, PhD
Stanford University

Get multiple results from just one sample

Achieve better cell proliferation data

Unlike the MTT assay, which only gives end-point data, HoloMonitor measures kinetic cellular characteristics at multiple time points in real-time. Hence, you can simultaneously compare the temporal effects of multiple treatments and/or conditions. Moreover, you can re-analyze your results with other HoloMonitor assays to generate more data without setting up new experiments.

Proliferation data with quantitative imaging

  • Real-time growth curves with or without normalization:
    • Cell count (cells/cm2)
    • Confluence (%)
  • Multiwell cell images and time-lapse videos
White cells on yellow background. Four panels showing a cell divide into two.

Get accurate data with digital holography

HoloMonitor uses digital holographic microscopy to collect publication-quality images without any labels. In addition, you receive accurate quantitative data. This way, you can study over 30 cell morphology parameters from a single sample and quantify cell behavior changes on both single-cell and population levels.

Bring Quantitative Imaging To Your Incubator

Label-free time-lapse video of cells proliferating over time imaged by HoloMonitor.

Publications with cell proliferation studies

Get inspired by other research fellows and learn how HoloMonitor quantitative live cell imaging can benefit your cell proliferation study

Four-day time-lapse quantitative phase imaging of cytokinesis failure in primary melanocytes transduced with MIR211-5p.

BRAFV600E induces reversible mitotic arrest in human melanocytes via microRNA-mediated suppression of AURKB

Authors: McNeal et al. Journal: eLife (2021)

In this study, to investigate why the same mutation has such different consequences in moles and melanomas, the authors using HoloMonitor studied cell proliferation, cell dry mass, cell death and cell growth rate. The experiments showed by increasing the levels of two microRNAs in melanocytes induces mitotic failure, genome duplication, and proliferation arrest. BRAFV600E induces a similar proliferation arrest in primary human melanocytes that is both reversible and conditional depending on the differentiation state of the melanocyte. Read more…

Targeted Delivery of Combination Therapeutics Using Monoclonal Antibody 2C5-Modified Immunoliposomes for Cancer Therapy

Authors: R. Narayanaswamy et al. Journal: Pharmaceutical Research (2021)

In this study, the authors have used monoclonal antibody-modified immunoliposomes for the targeted delivery of paclitaxel and salinomycin for cancer therapy. By using HoloMonitor M4, the authors measured the cell morphology, proliferation and cell division change between treatments, the results confirmed the efficacy of the antibody-targeted liposomal preparation in the therapy of cancer by giving significantly better cellular division arrest profiles and cell death. Read more…

Biomimetic dextran-based hydrogel layers for cell micropatterning over large areas using FluidFM BOT technology

Authors: Andras Saftics et al.

Journal: Langmuir (2019)

Research Areas: Biotechnology

Cell Lines: HeLa

Keywords: HoloMonitor M4, cell viability, cell proliferation, cell adhesion, cell migration, Biosensors, hydrogels

Learn more …

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

Authors: Jing Huang et al.

Journal: JoVe (video journal) (2018)

Research Areas: Cancer Research

Cell Lines: KHOS

Keywords: HoloMonitor M4, Cell morphology, Cell motility, Cell migration, Cell proliferation

Learn more …

Hydroxylated Fluorescent Dyes for Live-cell Labeling: Synthesis, Spectra and Super-resolution Sted

Authors: Dr. Alexey N. Butkevich et al.

Journal: Chemistry — A European Journal (2017)

Research Areas: Method development

Cell Lines: Drosophilia S2 cells

Keywords: HoloMonitor M4, Cell quality, Cell morphology, Cell proliferation

Learn more …

Label-free High Temporal Resolution Assessment of Cell Proliferation Using Digital Holographic Microscopy

Authors: Birgit Janicke et al.

Journal: Cytometry Part A (2017)

Research Areas: Method development

Cell Lines: L929

Keywords: HoloMonitor M4, Cell proliferation, Digital holographic microscopy, Cell count, Noninvasive, Label-free, Image cytometry, Quantitative phase imaging, Time-lapse, Digital holography technology

Learn more …

Biophysical Characteristics of Proteins and Living Cells Exposed to the Green Tea Polyphenol Epigallocatechin-3-gallate

Authors: Beatrix Peter et al.

Journal: European biophysics journal (2016)

Research Areas: Mode of action

Keywords: Review, Cell morphology, Cell motility, Cell proliferation, apoptosis

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Digital Holographic Microscopy for Non-invasive Monitoring of Cell Cycle Arrest in L929 Cells

Authors: Maria Falck Miniotis et al.

Journal: PLOS ONE (2014)

Research Areas: Method development

Cell Lines: L929

Keywords: Holomonitor M3, Cell morphology, Cell cycle and cell division, Flow cytometry, Cancer treatment, G1 phase, Colcemid treatment, Holographic microscopy, therapeutic drug

Learn more …