Malmö University

PHI and Malmö University have a long-standing collaboration, dating back to 2008. The collaboration has resulted in several peer reviewed publications and a doctoral thesis. In late 2016, the European Commission granted 2.1 million euro to GlycoImaging – a joint cancer research project to develop improved methods for clinically diagnosing cancer.

Current methods for diagnosing cancer primarily focus on the proteins associated with cancer. However, there is increasing evidence that carbohydrates play an important role in the development and progression of malignant cancer. Current methods use and rely on antibodies created by living organisms. These natural antibodies, however, are not sufficiently specific to accurately detect and image carbohydrates.

The GlycoImaging project is coordinated by Malmö University and commercialized by PHI. Additional partners are Bundesanstalt für Materialforschung und Prüfung (Germany’s federal technology research institute), Umeå, Copenhagen and Turku University.

Anette Gjörloff Wingren, Malmö University

Oncology research and diagnostics are in need of low-cost and robust probes to detect carbohydrates. The goal of the GlycoImaging project is to meet this need by combining specific carbohydrate probes – in the form of molecular imprinted polymers or ‘plastic antibodies’ – with holographic microscopy.

Prof. Anette Gjörloff Wingren

Faculty of Health and Society, Malmö University

Presentations

Popular lecture on cancer research by Prof. Anette Gjörloff Wingren 2016 (in Swedish).

A short presentation of GlycoImaging by Prof. Anette Gjörloff Wingren (in Swedish).

The GlycoImaging video (in Swedish).

Interviews and News

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Best Poster Prize to Louise Sternbaeck

PHI’s Industrial Doctoral Student Louise Stenbaeck received a prize from Danish Cancer Society for her poster “Holographic microscopy: Macrophage-uptake of SA-MIPs”.
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Digital Holographic Microscopy for biomarker detection in cancer

“In the near future”, Zahra El-Schich says, “you could have a HoloMonitor in every clinic. You could also customize cancer treatment for every patient by just using a simple tumor test.” Find out how.
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Fighting cancer at an early stage

Using nanoparticles and HoloMonitor technology Prof. Anette Gjörloff Wingren and her team aim to develop an early screening method that detects cancer by a simple blood test.
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PHI and Malmö University receives 2.3 million SEK to detect blood-borne cancer cells

GlycoImaging is a collaboration project between PHI, Malmö University and four international research institutions. By combining PHI’s HoloMonitor technology with a new type of cancer probes, more sensitive methods to detect and diagnose cancer at an earlier stage than what is possible today.
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EU grants 2.1 million euro to Phase Holographic Imaging and Malmö University with partners for joint cancer research

The European Commission has appointed Phase Holographic Imaging,Bundesanstalt für Material-forschung und Prüfung (Germany’s federal technology research institute), Malmö, Umeå, Copenhagen and Turku University to develop improved methods for clinically diagnosing cancer.
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Researchers propose PHI’s technology to assess reduction in cancer cell growth

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Peer Reviewed Articles and Book Chapters

Non-invasive, Label-free Cell Counting and Quantitative Analysis of Adherent Cells Using Digital Holography

Authors: A. Mölder et al.

Journal: Journal of Microscopy (2008)

Research Areas: Method development

Cell Lines: MCF-10 A, L929, PC-3, DU-145

Keywords: HoloMonitor M2, cell counting, confluence, phase shift, optical path length, digital holographic technology

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Digital Holographic Microscopy — Innovative and Non-destructive Analysis of Living Cells

Authors: Zahra El-Schich et al.

Journal: Microscopy: Science, Technology, Applications and Education (2010)

Research Areas: Method development

Keywords: engineering and technology, teknik och teknologier, digital holography, cell studies, cancer, viability, refractive index, digital holographic technology

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Digital Holography and Cell Studies

Authors: Kersti Alm et al.

Journal: Holography-Research and technologies (2011)

Research Areas: Method development

Keywords: Digital holographic technology

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Cells and Holograms — Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells

Authors: Kersti Alm et al.

Journal: Holography — Basic Principles and Contemporary Applications (2013)

Research Areas: Method development

Keywords: Digital holographic technology

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Digital Holographic Microscopy for Non-invasive Monitoring of Cell Cycle Arrest in L929 Cells

Authors: Maria Falck Miniotis et al.

Journal: PLOS ONE (2014)

Research Areas: Method development

Cell Lines: L929

Keywords: Holomonitor M3, Cell morphology, Cell cycle and cell division, Flow cytometry, Cancer treatment, G1 phase, Colcemid treatment, Holographic microscopy, therapeutic drug

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Interfacing Antibody-based Microarrays and Digital Holography Enables Label-free Detection for Loss of Cell Volume

Authors: Zahra El-Schich et al.

Journal: Future science oa (2015)

Research Areas: Cancer research, Drug research

Cell Lines: Jurkat, U2932

Keywords: HoloMonitor M2, cell counter, cell morphology, antibody, cellular, holography, microarray, volume, drug development

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Supervised Classification of Etoposide-treated in Vitro Adherent Cells Based on Noninvasive Imaging Morphology

Authors: Anna Leida Mölder et al.

Journal: Journal of Medical Imaging (2017)

Research Areas: Method development

Cell Lines: DU-145

Keywords: HoloMonitor M4, cell morphology, Image segmentation Image analysis In vitro testing, Digital holography Image classification, Microscopy, Biological research, Digital imaging, Holography, In vivo imaging

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Holography: the Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

Authors: Zahra El-Schich et al.

Journal: Holographic Materials and Optical Systems (2017)

Research Areas: Method development

Cell Lines: U2932, WM-266-4, CHL-1

Keywords: HoloMonitor M4, Cell death, Cell volume, Digital holographic, Microscopy, Individual treatment, Holography technology

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Quantitative Phase-contrast Imaging – a Potential Tool for Future Cancer Diagnostics

Authors: Anette Gjörloff-Wingren

Journal: Cytometry Part A (2017)

Research Areas: Method development

Keywords: Review

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Moving into a New Dimension: Tracking Migrating Cells with Digital Holographic Cytometry in 3D

Authors: Anette Görloff Wingren

Journal: Cytometry Part A (2018)

Research Areas: Cancer research

Keywords: HoloMonitor M4, cell motility, cell migration, cell morphology, cell tracking, cytometry, digital holography, migration, motility, quantitative phase imaging, scratch assay

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Digital Holographic Cytometry: Macrophage Uptake of Nanoprobes

Authors: Louise Sternbæk et al.

Journal: Imaging & Microscopy (2019)

Research Areas: Cytotoxicity

Cell Lines: RAW 264, 7

Keywords: HoloMonitor M4, Cytotoxicity, Cell morphology, Cell proliferation

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Evaluation of the Impact of Imprinted Polymer Particles on Morphology and Motility of Breast Cancer Cells by Using Digital Holographic Cytometry

Authors: Megha Patel et al.

Journal: Applied Sciences (2020)

Research Areas: Cancer research, Materials of Science

Cell Lines: MCF-7 and MDAMB231

Keywords: HoloMonitor M4, Cell morphology and cell movements, breast cancer, digital holographic cytometry, molecularly imprinted polymers, motility, sialic acid, viability

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Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Authors: Zahra El-Schich et al.

Journal: Applied Sciences (2020)

Research Areas: Cancer research

Cell Lines: Jurkat, THP-1, Hs-578T, MDA-MD-231, MDA-MB-468, T57D, Cama-1, MCF-7

Keywords: HoloMonitor M4, cell morphology, breast cancer, cell area, cell thickness, cell volume, circulating tumor cell, CD45, digital holographic cytometry, EpCAM

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Featured Applications

Label-free Cell Cycle Analysis

Live cell imaging cytometry of antibody captured suspension cells

Location

Faculty of Health and Society,
Malmö University
Jan Waldenströms gata 25, AS:F502
205 06 Malmö, Sweden

The Partnerships

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Lund University Cancer Centre

The early HoloMonitor development began at Lund University. The relationship between Lund University and PHI has since evolved into partnerships with several research groups.
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Uppsala University

Supported by PHI, scientists at Uppsala University apply state of the art artificial intelligence on its very source of inspiration — cell biology.
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UCSF

University of California in San Francisco, Gladstone Institutes and PHI jointly operate San Francisco’s Holographic Imaging Cytometry Center of Excellence.
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National Institute on Aging

In pursuit of making novel research tools available to National Institute on Aging and the medical research community in general, NIA and PHI are collaborating to develop a fluorescence module to PHI’s HoloMonitor.
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Gladstone Institutes

Gladstone Institutes, UCSF and PHI jointly operate San Francisco’s Holographic Imaging Cytometry Center of Excellence.
Commencement at University of Utah

Huntsman Cancer Institute

Supported by PHI, Dr. Robert Judson-Torres and his team at Huntsman Cancer Institute aim to develop methods to quantify the phenomena known as epithelial-mesenchymal transition (EMT).
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Northeastern University

The joint program of excellence between Northeastern University and PHI began in late 2014. Since then HoloMonitor technology has rapidly established itself as a technology of choice for label-free kinetic cellular analysis.