Advancing Cancer Research with Quantitative Live Cell Imaging
Cancer is a leading human disease with persistently high mortality rates. Thus, understanding the how, where, why and with whom metastatic cancer cells disseminate and proliferate is one of the highest importance in cancer research. The HoloMonitor live cell imaging system advances your cancer research with a non-invasive way to measure and quantify cell activity in real-time without compromising the integrity of your precious cells. Therefore, you can get more accurate and native results from your experiments.
A live cell analysis tool with real-time data
Find out more about how HoloMonitor live cell imaging can accelerate your cancer research!
Featured publications within cancer research
Compare directional cell movement in medulloblastoma cancer cells
The expression of long non-coding RNAs (lncRNAs) influences diverse cellular activities and is often cancer-specific. Thus lncRNAs are a useful diagnostic and therapeutic target for cancer. By altering the expression of lncRNA levels, it is possible to affect the cell movement in medulloblastoma cancer cells.
In this publication, the authors evaluated the effect of lncRNA on cell motility and showed that it promotes the migration of tumor cells using HoloMonitor wound healing assay and single cell tracking assay.
Measure mitotic activity in melanocytes
Cutaneous melanoma is deadly skin cancer. The activating mutation BARFV600E mutation exists both in moles and over half cutaneous melanomas.
In this study, in order to investigate why the same mutation has such different consequences in moles and melanomas, the authors using HoloMonitor studied cell proliferation, cell dry mass, cell death and cell growth rate. The experiments showed by increasing the levels of two microRNAs in melanocytes induces mitotic failure, genome duplication, and proliferation arrest. BRAFV600E induces a similar proliferation arrest in primary human melanocytes that is both reversible and conditional depending on the differentiation state of the melanocyte.
Are you interested in discussing non-invasive live cell imaging for your cancer research project?
Quantify cell motility under hypoxia stress in breast cancer
Hypoxia is an important factor known to initiate the metastatic cascade in cancer, activating cell motility and invasion by silencing cell adhesion genes.
In the study, the authors using cell motility assay demonstrated that epigenetic enzyme (G9a) accumulation in hypoxia leads to CDH10 protein transcription repression. This enhances cancer cell motility and is an integral part of hypoxia-mediated epithelial-mesenchymal transition (EMT) in breast cancer.
Study cell movements in 3D culture for cancer biomechanics
Studying the tumor microenvironment is one of the highest importance in cancer research as most tumors depend on a supporting cast of non-cancerous cells for their nourishment, growth and survival. 3D cell culturing mimics the actual physiological conditions in vivo than the traditional 2D cell cultures. This is particularly important when studying cell movements as the higher complexity of the microenvironment affect the biomechanics.
In this application note, the author used HoloMonitor single cell tracking and wound healing assay to quantify cell movement with 3D Matrigel cell cultures.
On-demand webinar
The secrets to truly controlled cell experiments for cancer research
Find out how HoloMonitor provides better insights by analyzing proliferation, morphology and movement data for both single cells and cell populations.
Discover HoloMonitor Live Cell Assays
Learn more about what you can do with HoloMonitor
Kinetic motility assay
Single cell tracking 
Cell morphology assay
Wound healing assay
Dose response assay Answer cancer research questions with quantitative live cell imaging
24 hours time-lapse video of MDA-MB-231 breast cancer cells
72 hours time-lapse video of JIMT-1 breast cancer cells
Browsing publications in cancer research
Get inspired by other research fellows, and learn how HoloMonitor quantitative live cell imaging can benefit your cancer research:
Interfacing Antibody-based Microarrays and Digital Holography Enables Label-free Detection for Loss of Cell Volume
Journal: Future science oa (2015)
Research Areas: Cancer research, Drug research
Cell Lines: Jurkat, U2932
Combination of Unsaturated Fatty Acids and Ionizing Radiation on Human Glioma Cells: Cellular, Biochemical and Gene Expression Analysis
Journal: Lipids in health and disease (2014)
Research Areas: Cancer research
Cell Lines: U87MG
The Role of Pi3k/akt-related Pip5k1α and the Discovery of Its Selective Inhibitor for Treatment of Advanced Prostate Cancer
Journal: PNAS (2014)
Research Areas: Cancer research
Cell Lines: PC-3
Protein kinase A (PKA) pathway is functionally linked to androgen receptor (AR) in the progression of prostate cancer
Journal: Urologic Oncology (2014)
Research Areas: Cancer research
Cell Lines: LNCaP, PC3
Lipids as Tumoricidal Components of Human Α-lactalbumin Made Lethal to Tumor Cells (Hamlet): Unique and Shared Effects on Signaling and Death
Journal: J. Of Biological Chemistry (2013)
Research Areas: Cancer research
Cell Lines: A549
A Unifying Mechanism for Cancer Cell Death Through Ion Channel Activation by Hamlet
Journal: PLOS One (2013)
Research Areas: Cancer research
Cell Lines: A549, HeLa, A498
The expansion of our existing collaboration with PHI is the result of productive utilization of HoloMonitor technology for non-invasive real-time cell cycle analysis in living cells. We are now developing state-of-the art applications multiplexing cell cycle and cell motility.
ED LUTHER
NORTHEASTERN UNIVERSITY